海带雌性原生质体的分离与培养     

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(S1):126-129

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。  相似文献   

Static Analysis of Collision Strength of Offshore Platform     

Li Runpei Chen Weigang Gu Yongning Professor  Shanghai Jiao Tong University  Shanghai Assistant  Shanghai Jiao Tong University  Shanghai 《中国海洋工程》1996,(1)

In this paper a numerical analysis method combining FEM incemental technique with limit analysis concept is proposed for the study of the static strength of offshore platform in collision. Large deformation and plasticity are accounted for and the limit yield surface expressed by generalized stress for a tubular section is derived. The modified stiffness matrix of space beam element is formulated by Plastic Node Method. The buckling behavior of beam columns can also be taken into account. It can trace the generation of plastic hinges during loading and finally the ultimate strength of offshore platform against collision is obtained.  相似文献   

Optimal Design for Main Dimensions of a 75000t Floating Production and Storage Vessel     

Tan Jiahua  Ma Wei  Pan Bin Associate Professor  Shanghai Jiao Tong University  Shanghai Lecturer  shanghai Jiao Tong University  Shanghai 《中国海洋工程》1992,(1)

The basis, process and results of the demonstration of the main dimensions of a 75000t floating production and storage vessel are discussed in this paper. A simple but reliable orthogonal design method is applied in the main dimension optimization. The ideas of gradual approximation and feedback from various aspects are put into effect. During the demonstration, in order to make the model tally with the actual situation, the draft design is closely related to the computational analysis, so that the demonstration model can be verified at any time; the handling of the overall system is closely related to the research of each item, which is beneficial not only to the mastery of various regularities, but also to the balance of decisions. Finally, according to the computational results and the regularities obtained from analysis, the main dimensions are determined.  相似文献   

Dynamic Analysis of a Single-Column Platform (II)     

Lian Gan  Ni Kan  Hu Kai Guo Dawei . Professor  Senior Engineer  Marine Design & Research Institute of China  Shanghai . Senior Engineer  Marine Design & REsearch Institute of China  Shanghai . Engineer  Marine Design & Research Institute of China  Shanghai 《中国海洋工程》1992,(4)

This paper describes the study of a single-column structure used as well-head platform. In order to check the reliability of computation theory and programme, model tests have been carried out. The paper introduces the conclusion of tests and the dynamic properties of single-column platform are obtained.  相似文献   

Elasto-Plastic Analysis of Tubular Joints of Offshore Platforms by Finite Element Method     

Chen Tieyun Zhu Zhenghong Professor  Dept. of Naval Architecture  Ocean Engineering  Shanghai Jiao Tong University  Shanghai Lecturer 《中国海洋工程》1991,(1)

The plastic node method is reformulated by the variational principle and is applied to elasto-plastic finite element analysis of tubular joints, eventually including the effect of internal and external gussets, stiffener rings, etc., if necessary. Four different joints are studied here in detail for the elasto-plastic behavior, the strain at the hot spot, the strain concentration factor around the intersection line, and the propagation of the plastic region with loading up to collapse in order to determine the ultimate strength, safety factor, and development of the plastic field. The present results are in good agreement with the experimental results.  相似文献   

科学大洋钻探与深部生物圈   总被引：1，自引：0，他引：1  

朱毅杰  于开平  周祖翼 《海洋地质前沿》2003,19(9):13-16,26

从深海钻探计划(DSDP)在20世纪70年代最早提出海洋沉积物中细菌活动性的证据到90年代大洋钻探计划(ODP)获得了令人信服的海底深部生物的存在证据，并成为即将启动的综合大洋钻探计划(IODP)的一个优先研究领域，深部生物圈成为科学大洋钻探近年来最为重大的发现之一，同时它也将有效地促进地球科学和生物学等其他学科的交叉。  相似文献   

辽东湾绥中海岸侵蚀研究   总被引：1，自引：0，他引：1  

王玉广  张宪文  贾凯  王权明 《海岸工程》2007,26(1):1-5

通过不同时期海图对比、分析遥感图像和多年现场监测资料,分析了辽东湾绥中沙质海岸侵蚀的特点,认为海岸组成物质松散与海岸动力作用强烈是本区现代海岸侵蚀的基本条件;陆源物质入海量减少、海动力作用、沿岸工程建筑和海上采砂是其海岸蚀退的主要影响因素。  相似文献   

动力定位系统与单波束测深仪在科考船定点作业中的应用     

张彬彬  张道建  张海泉  刘凯  张学贤  卢永平 《海洋通报》2020,39(6):651-656

科考船定点作业时会受到海洋风、涌、浪、流等外界环境因素影响，导致工作效率降低，原位测量精度下降，甚至影响作业安全。动力定位系统 （DP） 具有自动定位功能，能够抵抗外界环境因素的影响，可实现科考船高精度定点控位。 单波束测深仪不仅可以测量水深，也可反映水下设备深度信息，可以起到辅助监控水下设备功能。本文在介绍定点作业施工现状与局限性的基础上，分析 DP 系统与单波束测深仪工作原理，以“向阳红 01”船为载体，在定点作业时开启 DP 系统与单波束测深仪，发现该方法可以提高科考船定点作业工作效率、原位测量精度并保障作业安全，可为其他科考船定点作业提供参考。  相似文献   

Underice Operation of SJT-5 ROV     

Tang Rongqing  Wang Daoyan Zhang Xixian Egnineer  Shanghai Jiao Tong University  Shanghai  Associate Professor  Shanghai Jiao University  Shanghai 《中国海洋工程》1993,(3)

- The authors have used a low-cost ROV of observation type for underice surveying of the Nen-Jiang Cross-river oil pipe line in the Da Qing oil field. The operation method, process and the safety measures for severe cold areas are presented. The fruitful tries and experience may be useful to ROV operators in severe cold areas.  相似文献   

黄骅电厂取水河段取水特性的数模与物模研究     

童中山  应新亚  钱炳法 《海洋工程》1997,(4)

本文采用数值模拟与物模试验相结合的研究方法，详细地研究了黄骅电厂一、二、三期工程的取水特性。结果表明，现有河道只能满足电厂一期工程的取水需求；若将现有河道进行适当开挖，可以满足电厂三期取水需要。  相似文献